癌变·畸变·突变 ›› 2003, Vol. 15 ›› Issue (4): 205-208.doi: 10.3969/j.issn.1004-616x.2003.04.005

• 论著 • 上一篇    下一篇

药物AC-88对荷瘤鼠T细胞内TNF-α、lL-1β和IL-6 mRNA

马栋柱，孙克任，赵　丽，肖志峰，李　慧   

  1. 山东大学公共卫生学院毒理学研究室，山东　济南　250012
  • 收稿日期:2002-12-10 修回日期:2003-03-03 出版日期:2003-10-30 发布日期:2003-10-30
  • 通讯作者: 马栋柱

EFFECTS OF AC-88 ON TNF-α、 IL-1β、 IL-6 mRNA CONTENTS AND PROLIFERATION OF T LYMPHOCYTE IN TUMOUR BEARING MICE

MA Dong-zhu, SUN Ke-ren, ZHAO Li, et al   

  1. Department of Toxicology, Shandong University, Jinan 250012, China
  • Received:2002-12-10 Revised:2003-03-03 Online:2003-10-30 Published:2003-10-30
  • Contact: MA Dong-zhu

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摘要： 目的：探讨AC-88抗肿瘤作用及可能机制。方法：以移植H22肝癌细胞的小鼠为体内模型，研究了AC-88的抑瘤作用；应用细胞培养和MTT法研究荷瘤小鼠T淋巴细胞的增殖功能；用RT-PCR法研究荷瘤小鼠T淋巴细胞TNF-α、IL-1β和IL-6 mRNA含量变化。结果：实验发现，AC-88的体内抑瘤率可高达76 ％，而且AC-88能促进荷瘤鼠T淋巴细胞的增殖，能使T细胞中TNF-α、IL-1β、IL-6等细胞因子的mRNA含量增加2～4倍。　结论：AC-88有良好的抑瘤作用，还可以调节T淋巴细胞的功能，是一种具有较高开发价值的抗癌药物。

关键词: AC-88, 细胞因子, TNF-α, IL-1β, IL-6, T细胞增值

Abstract: Purpose: To investigate the antitumor effect and possible mechanism of AC-88. Methods: Tumor bearing mice were used as model in vivo to study the an titumor effect of AC-88. T cell proliferation was determined by using MTT method. The changes of TNF-α、 IL-1β、 IL-6 mRNA content in T cells were measured by RT-PCR. Results: The antitumor rate of AC-88 was 76 ％. Treatmemt with AC-88 significantly enhanced T cell proliferation, and increased contents of TNF-α,IL-1β,IL-6 mRNA in T cells by 2-4 folds compared with that in control(P< 0.01) . Conclusions: AC-88 can inhibit the growth of tumor and regulate the T cell function in tumor bearing mice, which is worthy to be developed into an anticarcinogen.

Key words: AC-88, cytokine, TNF -α, IL-1β, IL-6, T cell proliferation

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